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anti human ll 37 mouse monoclonal antibody  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology anti human ll 37 mouse monoclonal antibody
    Anti Human Ll 37 Mouse Monoclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 120 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti human ll 37 mouse monoclonal antibody/product/Santa Cruz Biotechnology
    Average 93 stars, based on 120 article reviews
    anti human ll 37 mouse monoclonal antibody - by Bioz Stars, 2026-03
    93/100 stars

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    Higher levels of <t>LL37</t> expression and NET release are determined in patients with SVV. (A) Telomere length of SVV and HCs by real-time Q-PCR; (B) levels of LL37 in serum from the disease group and healthy group; (C) levels of LL37 in urine from the disease group and healthy group; (D) levels of NET remnants in serum from patients with SVV or HCs; (E) levels of cf-DNA in serum from patients with SVV or HCs (A-E). N=70 for each group. The error bars of the graph represent the SEM. Unpaired Student’s t-test was conducted to analyze the statistical significance of two independent experiments; (F) an Alexa 488-labeled antibody against H2A-H2B-DNA complexes was used to visualize NETs in green, and DAPI was used to stain the nuclei in blue. Pictures were merged to form an overlay image; (G) H2A-H2B-DNA complexes, LL37, and nuclei are stained green, red, and blue, respectively. Pictures were merged to form an overlay image. A.U., arbitrary unit.
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    Higher levels of <t>LL37</t> expression and NET release are determined in patients with SVV. (A) Telomere length of SVV and HCs by real-time Q-PCR; (B) levels of LL37 in serum from the disease group and healthy group; (C) levels of LL37 in urine from the disease group and healthy group; (D) levels of NET remnants in serum from patients with SVV or HCs; (E) levels of cf-DNA in serum from patients with SVV or HCs (A-E). N=70 for each group. The error bars of the graph represent the SEM. Unpaired Student’s t-test was conducted to analyze the statistical significance of two independent experiments; (F) an Alexa 488-labeled antibody against H2A-H2B-DNA complexes was used to visualize NETs in green, and DAPI was used to stain the nuclei in blue. Pictures were merged to form an overlay image; (G) H2A-H2B-DNA complexes, LL37, and nuclei are stained green, red, and blue, respectively. Pictures were merged to form an overlay image. A.U., arbitrary unit.
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    Image Search Results


    Higher levels of LL37 expression and NET release are determined in patients with SVV. (A) Telomere length of SVV and HCs by real-time Q-PCR; (B) levels of LL37 in serum from the disease group and healthy group; (C) levels of LL37 in urine from the disease group and healthy group; (D) levels of NET remnants in serum from patients with SVV or HCs; (E) levels of cf-DNA in serum from patients with SVV or HCs (A-E). N=70 for each group. The error bars of the graph represent the SEM. Unpaired Student’s t-test was conducted to analyze the statistical significance of two independent experiments; (F) an Alexa 488-labeled antibody against H2A-H2B-DNA complexes was used to visualize NETs in green, and DAPI was used to stain the nuclei in blue. Pictures were merged to form an overlay image; (G) H2A-H2B-DNA complexes, LL37, and nuclei are stained green, red, and blue, respectively. Pictures were merged to form an overlay image. A.U., arbitrary unit.

    Journal: Annals of Translational Medicine

    Article Title: Telomere dysfunction promotes small vessel vasculitis via the LL37-NETs-dependent mechanism

    doi: 10.21037/atm.2020.02.130

    Figure Lengend Snippet: Higher levels of LL37 expression and NET release are determined in patients with SVV. (A) Telomere length of SVV and HCs by real-time Q-PCR; (B) levels of LL37 in serum from the disease group and healthy group; (C) levels of LL37 in urine from the disease group and healthy group; (D) levels of NET remnants in serum from patients with SVV or HCs; (E) levels of cf-DNA in serum from patients with SVV or HCs (A-E). N=70 for each group. The error bars of the graph represent the SEM. Unpaired Student’s t-test was conducted to analyze the statistical significance of two independent experiments; (F) an Alexa 488-labeled antibody against H2A-H2B-DNA complexes was used to visualize NETs in green, and DAPI was used to stain the nuclei in blue. Pictures were merged to form an overlay image; (G) H2A-H2B-DNA complexes, LL37, and nuclei are stained green, red, and blue, respectively. Pictures were merged to form an overlay image. A.U., arbitrary unit.

    Article Snippet: A FITC-labeled PNA probe specific for (TTAGGG)n sequences (PANAGENE, Korea) was co-denatured with the slide at 80 °C for 3 min and hybridized at RT for 2 h. Slides were then incubated first with the mouse-anti-human LL37 (HM2070, Hycult biotech, The Netherlands) and rabbit-anti-γH2AX (MABE205, Millipore, USA) antibodies, followed by the secondary Alexa-Fluor-594-labeled goat-anti-mouse antibody and Alexa-Fluor-488-labeled goat-anti-rabbit antibody.

    Techniques: Expressing, Labeling, Staining

    Immune aging of neutrophils and inflammatory medium release are determined in situ in individuals with SVV. (A) Neutrophils (polymorphic nuclear) infiltrated the kidney tissue from patients with SVV. Telomere dysfunction was found by the co-localization of γH2AX (purple) and telomeres (green) in the nucleus (DNA in blue). Immunostaining of LL37 showed perinuclear LL37 (red) in neutrophils that suffered DNA dysfunction; (B) H2A-H2B-DNA complexes, LL37, and nuclei are stained green, red, and blue, respectively. Pictures were merged to form an overlay image. LL37 assembled around the polymorphic nucleus in a background of NET deposition in the kidney tissue of SVV patients.

    Journal: Annals of Translational Medicine

    Article Title: Telomere dysfunction promotes small vessel vasculitis via the LL37-NETs-dependent mechanism

    doi: 10.21037/atm.2020.02.130

    Figure Lengend Snippet: Immune aging of neutrophils and inflammatory medium release are determined in situ in individuals with SVV. (A) Neutrophils (polymorphic nuclear) infiltrated the kidney tissue from patients with SVV. Telomere dysfunction was found by the co-localization of γH2AX (purple) and telomeres (green) in the nucleus (DNA in blue). Immunostaining of LL37 showed perinuclear LL37 (red) in neutrophils that suffered DNA dysfunction; (B) H2A-H2B-DNA complexes, LL37, and nuclei are stained green, red, and blue, respectively. Pictures were merged to form an overlay image. LL37 assembled around the polymorphic nucleus in a background of NET deposition in the kidney tissue of SVV patients.

    Article Snippet: A FITC-labeled PNA probe specific for (TTAGGG)n sequences (PANAGENE, Korea) was co-denatured with the slide at 80 °C for 3 min and hybridized at RT for 2 h. Slides were then incubated first with the mouse-anti-human LL37 (HM2070, Hycult biotech, The Netherlands) and rabbit-anti-γH2AX (MABE205, Millipore, USA) antibodies, followed by the secondary Alexa-Fluor-594-labeled goat-anti-mouse antibody and Alexa-Fluor-488-labeled goat-anti-rabbit antibody.

    Techniques: In Situ, Immunostaining, Staining

    Human neutrophils from SVV produce more LL37-mediated-NETs. (A) The percentage of spontaneously netting neutrophils from healthy controls (HCs) and SVV patients and the percentage of netting neutrophils after irradiation; (B) degree of NET release of neutrophils from HCs and SVV in the presence or absence of aprotinin; (C) degree of NET release of neutrophils after irradiation in the presence or absence of aprotinin. The graph is a minimum of 15 images derived from 3 independent experiments. The error bars of the graph represent SEM. Unpaired Student’s t-test was conducted to analyze the statistical significance of two independent experiments. ***, P<0.001; **, P<0.01; *, P<0.05; NS, not significant.

    Journal: Annals of Translational Medicine

    Article Title: Telomere dysfunction promotes small vessel vasculitis via the LL37-NETs-dependent mechanism

    doi: 10.21037/atm.2020.02.130

    Figure Lengend Snippet: Human neutrophils from SVV produce more LL37-mediated-NETs. (A) The percentage of spontaneously netting neutrophils from healthy controls (HCs) and SVV patients and the percentage of netting neutrophils after irradiation; (B) degree of NET release of neutrophils from HCs and SVV in the presence or absence of aprotinin; (C) degree of NET release of neutrophils after irradiation in the presence or absence of aprotinin. The graph is a minimum of 15 images derived from 3 independent experiments. The error bars of the graph represent SEM. Unpaired Student’s t-test was conducted to analyze the statistical significance of two independent experiments. ***, P<0.001; **, P<0.01; *, P<0.05; NS, not significant.

    Article Snippet: A FITC-labeled PNA probe specific for (TTAGGG)n sequences (PANAGENE, Korea) was co-denatured with the slide at 80 °C for 3 min and hybridized at RT for 2 h. Slides were then incubated first with the mouse-anti-human LL37 (HM2070, Hycult biotech, The Netherlands) and rabbit-anti-γH2AX (MABE205, Millipore, USA) antibodies, followed by the secondary Alexa-Fluor-594-labeled goat-anti-mouse antibody and Alexa-Fluor-488-labeled goat-anti-rabbit antibody.

    Techniques: Irradiation, Derivative Assay